A Study on Quantitative Estimation of Tannins in Mussaenda Erthyrophylla using Spectrophotometer

Abstract

Mussaenda erthyrophylla being an ornamental plant is still not fully explored. The most characterized compounds in Mussaendas are the iridoids, tannins triterpene and saponins. Very few species have been explored for chemical and biological studies. No pharmacognostic profile of the plant is available; its many allied species have been investigated for isolation and pharmacological activities. Saponins. a number of triterpenoids and glycosides were reported. Mussaenda genus viz., contains mussaendosides U(1) and V(2) , mussaendosides G(1) and K(2) are two new triterpenoid saponins , mussaendosides A-C, M and N with cyclolanostene type aglycone and aureusidin, iridoid glycosides. This has been adopted from Ethnobotanical Leaflets.Leaf, root contains tannins, astringents,  and mucilage.The aim of study is to estimate the amount of tannins present in the drug using folin-denis method. Mussaenda Erthyrophylla was crushed to coarse powder and extract with 50% aqueous methanol by cold maceration for 24h and make up volume upto 100ml.Tannin concentration was determined by the standard graph of tannic acid solution and was found to be 25.5 mg/g respectively. The concentration curve for tannic acid was determined and the correlation coefficient was calculated and was found to be 0.996 which indicates the good linearity between the concentration and the absorbance. It has been found that the tannins form the stable complexes with protein, starch and metal chelates by disturbing the metabolic activity of the bacterial enzymes nutrient availability, and functionality of biological membranes. The present study can be used as one of the parameters for standardization of medicinal plants