Comparative study of antibacterial efficacy of Ajwain seed oil and hexane extract against Escherichia coli

Abstract

Ajwain is an annual tropical aromatic herb widely known as Trachyspermum ammi (Apiaceae) also it is an annual plant which is widely cultivated in India, Iran and Persia. The fruits of T. ammi (Ajwain) traditionally were used as diuretic, carminative, and anthelmintic. Ajwain has been found to have numerous biological effects, including antibacterial, antiviral, antifungal, and antioxidant properties. Evaluating the antibacterial activity of hexane extract of seeds and essential oil against Escherichia coli bacterial strains was the purpose of this present research. After the preparation of the extracts and essential oil, the growth inhibitory zone assay was used to evaluate the antibacterial activity utilizing the disc diffusion, weight variation, and colony counting methods. Minimum inhibitory concentration (MIC) was measured by comparing results of growth inhibitory zone assay. The Amoxicillin stock solution (1 mg/ml) for zone of inhibition served as the benchmark for the comparative evaluation. The different outcome data was examined and results were compared among each other. After conducting a thorough analysis, we have determined that ajwain essential oil exhibits greater antibacterial activity as compared to ajwain seed extract.