RECENT ADVANCEMENT ON ISOLATION, ACTIVATION AND CRYOPRESERVATION OF LYMPH NODE CELLS IN MICE.

Abstract

ABSTRACT                                                                                                                                     

Lymph nodes are found within the body has B, T and other immune cells and help to filter and trap foreign particles. Like any other primary culture lymph node culture would retain many of differentiated characteristics of cells in vivo thus they have potential for acting as alternative method to mammalian model.

For setting up primary lymph node culture in mice different types of lymph nodes were collected from mice followed with isolation, activation and cryopreservation of cells from lymph node. The present review emphasize on various procedures used for isolation, activation and cryopreservation of lymph node cells. Isolation of cells was performed by collagenase digestion, teasing apart of lymph node using dissecting needle or lymph nodes were disrupted between two frosted slides. Concanavalin A have been widely used to stimulate mice lymph node cells. Low dose of Con A have stimulatory effect on T cells but high dose have inhibitory action and caused suppression of proliferation of T cell. Balb/c mice and C57Bl/6 mice were used for different dose of Con A. The addition of cryoprotective agents, e.g.dimethylsulphoxide and careful control of cooling rates affords protection from cell damage during freezing.