Evaluation of Total Phenolic Content, Flavonoid Content and In-vitro Free Radical Scavenging Activity of Solanum indicum Linn.

Abstract

Objective: To evaluate total phenolic contents, flavonoid contents and in-vitro free radical scavenging activity of
methanolic extract of leaves of Solanum indicum Linn.
Methods: Phytochemical screening, qualitative, quantitative determination of total phenol content (TPC), total flavonoid content (TFC), and in-vitro free radical scavenging activity such as DPPH, hydrogen Peroxide (H2O2) and reducing power assay (RPA) were performed using standard procedures.
Results: Phytochemical analysis of methanolic extract revealed the presence of important classes of phytochemicals such as tannins, carbohydrates, alkaloids, terpenoids, glycosides, flavonoids, and others. The total phenolic and flavonoid content of methanolic extract of S. indicum leaves was found to be the highest as 101 ± 0.72 and 97.19 ± 2.39 mg/gm of the standard drugs. In in-vitro DPPH free radical scavenging analysis, the IC50 methanolic extract was found to be 241.6 μg/mL as compared to standards Ascorbic acid (132.7 μg/mL) and BHT (177.81 μg/mL). In contrast, in hydrogen peroxide scavenging analysis, the IC50 value were found to be 24.30 μg/mL for methanolic extract. In contrast, for standards, it was found to be 21.30 and 23.67 μg/mL for ascorbic acid and BHT, respectively.
Conclusion: According to our findings, the methanolic extract of S. indicum leaves is a more powerful antioxidant in comparison with the aqueous extract of S. indicum leaves. For this reason present study suggested that leaves of S. indicum are a potential source of natural antioxidants. Further study is needed to identify the chemical compounds for various pharmacological attributes.