INTERNATIONAL JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH (IJPER)

RP-HPLC Method Development and Validation for the Estimation of Antidiabetic Drugs Remogliflozin Etabonate and Vildagliptin in Bulk and Pharmaceutical Dosage Form

2024-10-17T09:31:27+02:00

Introduction: The present study was done to develop a rapidand precise method by using reverse-phase high-performance liquid chromatography.
Materials and Methods: This method was developed for the validation of Remogliflozin etabonate and vildagliptin in pharmaceutical dosage forms. Chromatography was carried out on a hypersil C18 (4.6×150 mm, 5 μm) column using a mixture of acetonitrile: Water (50:50% v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection was carried out at 259 nm.
Results: The retention times of remogliflozin etabonate and vildagliptin were found to be 2.344 and 3.282 minutes, respectively. This method produced linear responses in the concentration range of 10 to 50 ppm of remogliflozin etabonate 5 to 25 ppm for vildagliptin. The precision for the determination of assay was below 2.0% RSD.
Discussion: This method can be used in the quality control tests of both bulk and pharmaceutical formulations.

Evaluation of Total Phenolic Content, Flavonoid Content and In-vitro Free Radical Scavenging Activity of Solanum indicum Linn.

2024-10-17T09:31:27+02:00

Objective: To evaluate total phenolic contents, flavonoid contents and in-vitro free radical scavenging activity of
methanolic extract of leaves of Solanum indicum Linn.
Methods: Phytochemical screening, qualitative, quantitative determination of total phenol content (TPC), total flavonoid content (TFC), and in-vitro free radical scavenging activity such as DPPH, hydrogen Peroxide (H2O2) and reducing power assay (RPA) were performed using standard procedures.
Results: Phytochemical analysis of methanolic extract revealed the presence of important classes of phytochemicals such as tannins, carbohydrates, alkaloids, terpenoids, glycosides, flavonoids, and others. The total phenolic and flavonoid content of methanolic extract of S. indicum leaves was found to be the highest as 101 ± 0.72 and 97.19 ± 2.39 mg/gm of the standard drugs. In in-vitro DPPH free radical scavenging analysis, the IC50 methanolic extract was found to be 241.6 μg/mL as compared to standards Ascorbic acid (132.7 μg/mL) and BHT (177.81 μg/mL). In contrast, in hydrogen peroxide scavenging analysis, the IC50 value were found to be 24.30 μg/mL for methanolic extract. In contrast, for standards, it was found to be 21.30 and 23.67 μg/mL for ascorbic acid and BHT, respectively.
Conclusion: According to our findings, the methanolic extract of S. indicum leaves is a more powerful antioxidant in comparison with the aqueous extract of S. indicum leaves. For this reason present study suggested that leaves of S. indicum are a potential source of natural antioxidants. Further study is needed to identify the chemical compounds for various pharmacological attributes.

Applications of Various Software in Pharmaceuticals: A Review

2024-10-17T09:31:27+02:00

To address issues with current good manufacturing practices (cGMP), production, documentation, regulatory body requirements (US, FDA, WHO, EU-GMP, PICS), product quality, and other issues, the pharmaceutical industry as a whole needed a variety of creative and scientific solutions. One key tool in addressing these issues is computer system software, which monitors and upholds current industry practices. Installing such software will assist the pharmaceutical sector in meeting regulatory body standards and enhancing its cGMP processes. The pharmaceutical industry’s recent advancements have increased the complexity of products and data. In the pharmaceutical sector, several elements are considered simultaneously. As a result, several complex software applications are used today to control these factors. Software is a group of programs that define the tasks that a computer must perform. It is made up of a series of programming language-written, computer-interpreted code. It thus makes our jobs easier by maintaining all the records, doing all the necessary computations, and much more. The pharmaceutical industry uses a broad variety of software since the tasks that need to be accomplished are equally varied. Regulatory compliance management, quality control test results management, marketing and sales campaign data handling, record-keeping for a batch of tablets in the manufacturing industry, etc. An overview of the many software categories utilized in the pharmaceutical sector is provided in this article. It also discusses their significance and needs in the pharmaceutical sector. It also discusses the significance of software utilized in the pharmaceutical sector.

Exploring the Efficacy of Sulphonamide in Novel Therapeutic Approaches: New Hope

2024-10-17T09:31:27+02:00

Sulphonamides are widely used in medicine for their antibacterial, antifungal, antiviral, antitumor, anti-inflammatory, and carbonic anhydrase-inhibiting properties. They can block protein and nucleic acid production in bacteria. Adding aromatic heterocycles like benzotriazole and benzimidazole to sulphonamides can enhance their antibacterial potency. Fluoro- and chloro-substituted benzyl groups have also been added to improve pharmacological properties. Sulphonamides can react with and ketones to form Schiff bases, which have antimicrobial and antimycotic properties. It has applications as an antimicrobial agent, hypoglycemic agent, diuretic, and more. The therapeutic actions of sulphonamides involve inhibiting enzymes like carbonic anhydrases, cyclooxygenases, and dihydropteroate synthetase. This allows them to have antimicrobial, anti-inflammatory, aldehydes, antiprotozoal and other effects. Sulphonamides like acetazolamide, celecoxib, zonisamide, and benzolamide have wide clinical applications as diuretics, anticancer agents, antiepileptics, anti-inflammatories, and more. Research is ongoing into synthesizing new sulphonamide derivatives with improved efficacy, potency, and safety profiles for treating various diseases.